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cultrex reduced growth factor basement membrane matrix  (R&D Systems)


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    R&D Systems cultrex reduced growth factor basement membrane matrix
    Cultrex Reduced Growth Factor Basement Membrane Matrix, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+matrix/Cultrex+RGF+Basement+Membrane+Extract/pm41921755-121-3-10
    Average 95 stars, based on 54 article reviews
    cultrex reduced growth factor basement membrane matrix - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Sterility:

    Article Title: Suspended hydrogel culture as a method to scale up intestinal organoids.
    Article Snippet: .. Crypts were pelleted at 450 × g for 5 min, washed in PBS, filtered through sterile gauze then a 100 μm cell strainer to remove debris, and pelleted at 450 × g for 5 min. Crypts were resuspended in Cultrex Reduced Growth Factor Basement Membrane Matrix, Type II (BME, R&D Systems cat. no. 3533-010-02) on ice, plated in 50 μL domes in a 24-well plate, cured at 37 °C for 15–30 min, then overlaid with 500 μL Colon Passage Media (Intesticult Organoid Growth Medium (OGM, StemCell Technologies cat. no. 06010) + 10 μM Y-27632) or Ileum Media (OGM + 10 μM Y-27632 + 2.5 μM CHIR99021). ..

    Article Title: Suspended hydrogel culture as a method to scale up intestinal organoids
    Article Snippet: .. Crypts were pelleted at 450 × g for 5 min, washed in PBS, filtered through sterile gauze then a 100 μm cell strainer to remove debris, and pelleted at 450 × g for 5 min. Crypts were resuspended in Cultrex Reduced Growth Factor Basement Membrane Matrix, Type II (BME, R&D Systems cat. no. 3533-010-02) on ice, plated in 50 μL domes in a 24-well plate, cured at 37 °C for 15–30 min, then overlaid with 500 μL Colon Passage Media (Intesticult Organoid Growth Medium (OGM, StemCell Technologies cat. no. 06010) + 10 μM Y-27632) or Ileum Media (OGM + 10 μM Y-27632 + 2.5 μM CHIR99021). ..

    Membrane:

    Article Title: Suspended hydrogel culture as a method to scale up intestinal organoids.
    Article Snippet: .. Crypts were pelleted at 450 × g for 5 min, washed in PBS, filtered through sterile gauze then a 100 μm cell strainer to remove debris, and pelleted at 450 × g for 5 min. Crypts were resuspended in Cultrex Reduced Growth Factor Basement Membrane Matrix, Type II (BME, R&D Systems cat. no. 3533-010-02) on ice, plated in 50 μL domes in a 24-well plate, cured at 37 °C for 15–30 min, then overlaid with 500 μL Colon Passage Media (Intesticult Organoid Growth Medium (OGM, StemCell Technologies cat. no. 06010) + 10 μM Y-27632) or Ileum Media (OGM + 10 μM Y-27632 + 2.5 μM CHIR99021). ..

    Article Title: SETD1A regulates psychiatric gene networks involved in genomic stability and synaptic function in rare and sporadic schizophrenia
    Article Snippet: .. iPSC lines were maintained on a tissue culture plate coated with Cultrex Reduced Growth Factor Basement Membrane Matrix (R&D Systems) in StemFlex medium (Thermo Fisher Scientific). .. To generate isogenic SETD1A mutant lines, an oligonucleotide (sgRNA) targeting around the splice acceptor of intron 15 in SETD1A was designed and cloned into U6-gRNA:CMV-CAS9-2A-tGFP vector by MilliporeSigma.

    Article Title: Acetoacetate suppresses colon cancer via an MR1-MAIT axis
    Article Snippet: .. For orthotopic tumor transplantations, tumor colonoids (cultured as described below) were dissociated with TrypLE Express (Thermo Fisher Scientific) for 5 min at 37°C, resuspended in basal medium containing 10% Cultrex Reduced Growth Factor Basement Membrane Matrix (BME; R&D Systems) and injected into the colonic mucosa of recipient mice (8-12 weeks old) by colonoscopy as previously described . .. Briefly, a Karl Storz endoscope system consisting of a documentation system (AIDA), cold light source (Xenon 175), imaging system (Image1 S; all Karl Storz), and a Hopkins telescope (1232 AA) were used.

    Article Title: The differentiation state of small intestinal organoid models influences prediction of drug-induced toxicity
    Article Snippet: .. Crypts were collected in Advanced DMEM/F12 with Primocin and filtered through a 500 μm strainer, then resuspended in Cultrex Reduced Growth Factor Basement Membrane Matrix, Type II (BME, cat. no. 3533-01002; R&D Systems) on ice and cultured in 50 μL/well domes in a 24-well plate (Corning). .. The domes were cured at 37°C for at least 10 min then overlaid with 0.5 mL passage medium consisting of IntestiCult Human Intestinal Organoid Growth Medium (OGM, cat. no. 06010; STEMCELL Technologies) supplemented with 0.1 mg/mL Primocin, 10 μM ROCK inhibitor Y-27632 (cat. no. 72304; STEMCELL Technologies) and 2.5 μM GSK-3 inhibitor CHIR 99021 (cat. no. 4423; Tocris Bioscience).

    Article Title: Suspended hydrogel culture as a method to scale up intestinal organoids
    Article Snippet: .. Crypts were pelleted at 450 × g for 5 min, washed in PBS, filtered through sterile gauze then a 100 μm cell strainer to remove debris, and pelleted at 450 × g for 5 min. Crypts were resuspended in Cultrex Reduced Growth Factor Basement Membrane Matrix, Type II (BME, R&D Systems cat. no. 3533-010-02) on ice, plated in 50 μL domes in a 24-well plate, cured at 37 °C for 15–30 min, then overlaid with 500 μL Colon Passage Media (Intesticult Organoid Growth Medium (OGM, StemCell Technologies cat. no. 06010) + 10 μM Y-27632) or Ileum Media (OGM + 10 μM Y-27632 + 2.5 μM CHIR99021). ..

    Article Title: SETD1A regulates psychiatric gene networks involved in genomic stability and synaptic function in rare and sporadic schizophrenia.
    Article Snippet: .. Generation of isogenic SETD1A LoF mutant iPSCs. iPSC lines were maintained on a tissue culture plate coated with Cultrex Reduced Growth Factor Basement Membrane Matrix (R&D Systems) in StemFlex medium (Thermo Fisher Scientific). .. To generate isogenic SETD1A mutant lines, an oligonucleotide (sgRNA) targeting around the splice acceptor of intron 15 in SETD1A was designed and cloned into U6-gRNA:CMV-CAS9-2A-tGFP vector by MilliporeSigma.

    Article Title: Phosphatidylserine-Rich circulating extracellular vesicles activate TAM receptor signaling to promote skin wound repair.
    Article Snippet: Phosphatidylserine (PS) is an anionic phospholipid that acquires signaling activity when exposed on membrane surfaces.. Although small extracellular vesicles (sEVs) have been implicated in diverse biological processes, the contribution of membrane-associated PS to vesicle-mediated signaling remains incompletely understood.. Here, we show that circulating small extracellular vesicles (csEVs) activate dermal fibroblasts and keratinocytes through a PS-dependent receptor signaling mechanism.

    Article Title: Acetoacetate suppresses colon cancer via an MR1-MAIT axis
    Article Snippet: .. Briefly, AKP organoids were embedded 10 μL per dome in Cultrex Reduced Growth Factor Basement Membrane Matrix (BME; R&D Systems) and cultured in basal medium consisting of Advanced DMEM/F12 (Gibco) supplemented with 1% penicillin-streptomycin, GlutaMAX (Gibco), and 5% FBS. ..

    Cell Culture:

    Article Title: Acetoacetate suppresses colon cancer via an MR1-MAIT axis
    Article Snippet: .. For orthotopic tumor transplantations, tumor colonoids (cultured as described below) were dissociated with TrypLE Express (Thermo Fisher Scientific) for 5 min at 37°C, resuspended in basal medium containing 10% Cultrex Reduced Growth Factor Basement Membrane Matrix (BME; R&D Systems) and injected into the colonic mucosa of recipient mice (8-12 weeks old) by colonoscopy as previously described . .. Briefly, a Karl Storz endoscope system consisting of a documentation system (AIDA), cold light source (Xenon 175), imaging system (Image1 S; all Karl Storz), and a Hopkins telescope (1232 AA) were used.

    Article Title: The differentiation state of small intestinal organoid models influences prediction of drug-induced toxicity
    Article Snippet: .. Crypts were collected in Advanced DMEM/F12 with Primocin and filtered through a 500 μm strainer, then resuspended in Cultrex Reduced Growth Factor Basement Membrane Matrix, Type II (BME, cat. no. 3533-01002; R&D Systems) on ice and cultured in 50 μL/well domes in a 24-well plate (Corning). .. The domes were cured at 37°C for at least 10 min then overlaid with 0.5 mL passage medium consisting of IntestiCult Human Intestinal Organoid Growth Medium (OGM, cat. no. 06010; STEMCELL Technologies) supplemented with 0.1 mg/mL Primocin, 10 μM ROCK inhibitor Y-27632 (cat. no. 72304; STEMCELL Technologies) and 2.5 μM GSK-3 inhibitor CHIR 99021 (cat. no. 4423; Tocris Bioscience).

    Article Title: Acetoacetate suppresses colon cancer via an MR1-MAIT axis
    Article Snippet: .. Briefly, AKP organoids were embedded 10 μL per dome in Cultrex Reduced Growth Factor Basement Membrane Matrix (BME; R&D Systems) and cultured in basal medium consisting of Advanced DMEM/F12 (Gibco) supplemented with 1% penicillin-streptomycin, GlutaMAX (Gibco), and 5% FBS. ..

    Injection:

    Article Title: Acetoacetate suppresses colon cancer via an MR1-MAIT axis
    Article Snippet: .. For orthotopic tumor transplantations, tumor colonoids (cultured as described below) were dissociated with TrypLE Express (Thermo Fisher Scientific) for 5 min at 37°C, resuspended in basal medium containing 10% Cultrex Reduced Growth Factor Basement Membrane Matrix (BME; R&D Systems) and injected into the colonic mucosa of recipient mice (8-12 weeks old) by colonoscopy as previously described . .. Briefly, a Karl Storz endoscope system consisting of a documentation system (AIDA), cold light source (Xenon 175), imaging system (Image1 S; all Karl Storz), and a Hopkins telescope (1232 AA) were used.

    Mutagenesis:

    Article Title: SETD1A regulates psychiatric gene networks involved in genomic stability and synaptic function in rare and sporadic schizophrenia.
    Article Snippet: .. Generation of isogenic SETD1A LoF mutant iPSCs. iPSC lines were maintained on a tissue culture plate coated with Cultrex Reduced Growth Factor Basement Membrane Matrix (R&D Systems) in StemFlex medium (Thermo Fisher Scientific). .. To generate isogenic SETD1A mutant lines, an oligonucleotide (sgRNA) targeting around the splice acceptor of intron 15 in SETD1A was designed and cloned into U6-gRNA:CMV-CAS9-2A-tGFP vector by MilliporeSigma.



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